Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.

Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.

Blog Article

We present a fast, reliable and inexpensive restriction-free cloning method for seamless SLEEP DNA insertion into any plasmid without sequence limitation.Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day.We show that EMP Queen LP Foundation cloning has a higher efficiency than restriction-free (RF) cloning, especially for long inserts above 2.5 kb.EMP further enables simultaneous cloning of multiple inserts.